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t cell medium  (TaKaRa)


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    Structured Review

    TaKaRa t cell medium
    T Cell Medium, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/t+cell+medium/LymphoONE+T-Cell+Expansion+Xeno-Free+Medium/10__1158_slash_2326___6066__cir___25___0605-138-19-27
    Average 93 stars, based on 27 article reviews
    t cell medium - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: T cells expressing a LMP1-specific chimeric antigen receptor mediate antitumor effects against LMP1-positive nasopharyngeal carcinoma cells in vitro and in vivo
    Article Snippet: .. The HELA/CAR lentivirus coated wells were seeded with 1×10 6 activated T cells at a concentration of 0.5×10 6 cell/mL in T cell medium (GT-T551, Takara, Japan) plus 100 U/mL of IL-2. ..

    Article Title: T cells expressing a LMP1-specific chimeric antigen receptor mediate antitumor effects against LMP1-positive nasopharyngeal carcinoma cells in vitro and in vivo
    Article Snippet: .. The HELA/CAR lentivirus coated wells were seeded with 16106 activated T cells at a concentration of 0.56106 cell/mL in T cell medium (GT-T551, Takara, Japan) plus 100 U/mL of IL2. ..

    Flow Cytometry:

    Article Title: Increased Lactate in Gastric Cancer Tumor-Infiltrating Lymphocytes Is Related to Impaired T Cell Function Due to miR-34a Deregulated Lactate Dehydrogenase A.
    Article Snippet: The sections were stained with diaminobenzidine, mounted on slides, and photographed using a digital microscope camera (Nikon, Tokyo, Japan). .. Flow cytometry detection The magnetic beads isolated T cells were maintained in the T cell medium (GT-T551 Medium for T-Cell Culture and Expansion; TaKaRa) and stimulated with Leukocyte Activation Cocktail, with BD GolgiPlugTM (BD Pharmingen) for 4 h. The cells were fixed and permeabilized with Fixation and Permeabilization Solution (BD Pharmingen). .. The cells were analyzed with a LIVE-OR-DYETM 350/448 Fixable Viability Staining Kit (Thermo Fisher) and stained using antibodies against: CD4 (PE, Mouse, Anti-Human, CD4, 555347), CD8 (FITC, Mouse, Anti-Human, CD8, 560960), and IFN-γ (APC Mouse Anti-Human IFN-γ Clone B27).

    Magnetic Beads:

    Article Title: Increased Lactate in Gastric Cancer Tumor-Infiltrating Lymphocytes Is Related to Impaired T Cell Function Due to miR-34a Deregulated Lactate Dehydrogenase A.
    Article Snippet: The sections were stained with diaminobenzidine, mounted on slides, and photographed using a digital microscope camera (Nikon, Tokyo, Japan). .. Flow cytometry detection The magnetic beads isolated T cells were maintained in the T cell medium (GT-T551 Medium for T-Cell Culture and Expansion; TaKaRa) and stimulated with Leukocyte Activation Cocktail, with BD GolgiPlugTM (BD Pharmingen) for 4 h. The cells were fixed and permeabilized with Fixation and Permeabilization Solution (BD Pharmingen). .. The cells were analyzed with a LIVE-OR-DYETM 350/448 Fixable Viability Staining Kit (Thermo Fisher) and stained using antibodies against: CD4 (PE, Mouse, Anti-Human, CD4, 555347), CD8 (FITC, Mouse, Anti-Human, CD8, 560960), and IFN-γ (APC Mouse Anti-Human IFN-γ Clone B27).

    Isolation:

    Article Title: Increased Lactate in Gastric Cancer Tumor-Infiltrating Lymphocytes Is Related to Impaired T Cell Function Due to miR-34a Deregulated Lactate Dehydrogenase A.
    Article Snippet: The sections were stained with diaminobenzidine, mounted on slides, and photographed using a digital microscope camera (Nikon, Tokyo, Japan). .. Flow cytometry detection The magnetic beads isolated T cells were maintained in the T cell medium (GT-T551 Medium for T-Cell Culture and Expansion; TaKaRa) and stimulated with Leukocyte Activation Cocktail, with BD GolgiPlugTM (BD Pharmingen) for 4 h. The cells were fixed and permeabilized with Fixation and Permeabilization Solution (BD Pharmingen). .. The cells were analyzed with a LIVE-OR-DYETM 350/448 Fixable Viability Staining Kit (Thermo Fisher) and stained using antibodies against: CD4 (PE, Mouse, Anti-Human, CD4, 555347), CD8 (FITC, Mouse, Anti-Human, CD8, 560960), and IFN-γ (APC Mouse Anti-Human IFN-γ Clone B27).

    Article Title: FOXM1-specific TCR-engineered T cells target non-small cell lung cancer
    Article Snippet: PBMCs were used to isolate CD8+ T cells by negative selection using Human CD8+ T cell Isolation kit (Stemcell, 17953). .. Isolated CD8+ T cells were activated by culturing T cells in T-cell medium (LymphoOneTM T cell expansion medium, Takara, WK552S) with T cell TransAct (Miltenyi Biotec, 130-111- 160) and 100U/ml of recombinant human IL-2 (PeproTech, 200-02-250) for 48 hours. .. 24-well non-tissue culture treated plates were coated with 20ug/ml of RetroNectin (Takara, T110B) in 4C overnight.

    Activation Assay:

    Article Title: Increased Lactate in Gastric Cancer Tumor-Infiltrating Lymphocytes Is Related to Impaired T Cell Function Due to miR-34a Deregulated Lactate Dehydrogenase A.
    Article Snippet: The sections were stained with diaminobenzidine, mounted on slides, and photographed using a digital microscope camera (Nikon, Tokyo, Japan). .. Flow cytometry detection The magnetic beads isolated T cells were maintained in the T cell medium (GT-T551 Medium for T-Cell Culture and Expansion; TaKaRa) and stimulated with Leukocyte Activation Cocktail, with BD GolgiPlugTM (BD Pharmingen) for 4 h. The cells were fixed and permeabilized with Fixation and Permeabilization Solution (BD Pharmingen). .. The cells were analyzed with a LIVE-OR-DYETM 350/448 Fixable Viability Staining Kit (Thermo Fisher) and stained using antibodies against: CD4 (PE, Mouse, Anti-Human, CD4, 555347), CD8 (FITC, Mouse, Anti-Human, CD8, 560960), and IFN-γ (APC Mouse Anti-Human IFN-γ Clone B27).

    Recombinant:

    Article Title: FOXM1-specific TCR-engineered T cells target non-small cell lung cancer
    Article Snippet: PBMCs were used to isolate CD8+ T cells by negative selection using Human CD8+ T cell Isolation kit (Stemcell, 17953). .. Isolated CD8+ T cells were activated by culturing T cells in T-cell medium (LymphoOneTM T cell expansion medium, Takara, WK552S) with T cell TransAct (Miltenyi Biotec, 130-111- 160) and 100U/ml of recombinant human IL-2 (PeproTech, 200-02-250) for 48 hours. .. 24-well non-tissue culture treated plates were coated with 20ug/ml of RetroNectin (Takara, T110B) in 4C overnight.

    Article Title: FOXM1-specific TCR-engineered T cells target non-small cell lung cancer
    Article Snippet: .. Antigen-specific T cells were generated with autologous PBMCs mixed with irradiated, peptide pulsed DCs at 35:1 ratio cultured in T cell medium (LymphoOneTM T cell expansion medium, Takara, WK552S) supplemented with 5ng/ml of recombinant D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -25-0605/3748919/cir-25-0605.pdf by guest on 22 M arch 2026 Bontekoe, E., Zhang, M., et al. 17 human IL-7 (R&D, 207-IL-005), and 30ng/ml of recombinant human IL-21 (PeproTech, AF-200-21) for one week to enhance growth of antigen-specific T cells. ..

    Generated:

    Article Title: FOXM1-specific TCR-engineered T cells target non-small cell lung cancer
    Article Snippet: .. Antigen-specific T cells were generated with autologous PBMCs mixed with irradiated, peptide pulsed DCs at 35:1 ratio cultured in T cell medium (LymphoOneTM T cell expansion medium, Takara, WK552S) supplemented with 5ng/ml of recombinant D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -25-0605/3748919/cir-25-0605.pdf by guest on 22 M arch 2026 Bontekoe, E., Zhang, M., et al. 17 human IL-7 (R&D, 207-IL-005), and 30ng/ml of recombinant human IL-21 (PeproTech, AF-200-21) for one week to enhance growth of antigen-specific T cells. ..

    Irradiation:

    Article Title: FOXM1-specific TCR-engineered T cells target non-small cell lung cancer
    Article Snippet: .. Antigen-specific T cells were generated with autologous PBMCs mixed with irradiated, peptide pulsed DCs at 35:1 ratio cultured in T cell medium (LymphoOneTM T cell expansion medium, Takara, WK552S) supplemented with 5ng/ml of recombinant D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -25-0605/3748919/cir-25-0605.pdf by guest on 22 M arch 2026 Bontekoe, E., Zhang, M., et al. 17 human IL-7 (R&D, 207-IL-005), and 30ng/ml of recombinant human IL-21 (PeproTech, AF-200-21) for one week to enhance growth of antigen-specific T cells. ..

    Cell Culture:

    Article Title: FOXM1-specific TCR-engineered T cells target non-small cell lung cancer
    Article Snippet: .. Antigen-specific T cells were generated with autologous PBMCs mixed with irradiated, peptide pulsed DCs at 35:1 ratio cultured in T cell medium (LymphoOneTM T cell expansion medium, Takara, WK552S) supplemented with 5ng/ml of recombinant D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -25-0605/3748919/cir-25-0605.pdf by guest on 22 M arch 2026 Bontekoe, E., Zhang, M., et al. 17 human IL-7 (R&D, 207-IL-005), and 30ng/ml of recombinant human IL-21 (PeproTech, AF-200-21) for one week to enhance growth of antigen-specific T cells. ..



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    Intracranial tumor transplantation experiment. (A) Survival duration of normal mice, mice subjected to cranial irradiation, mice with solely transplanted tumors, and mice with transplanted tumors following cranial irradiation (n=6). (B) The MRI scan clearly illustrates the size of the tumor, and the tumor is outlined by a red dashed line. (C) IHC of CD4, <t>CD8,</t> CD56, PD1, and Ki67 in peritumoral tissue and tumor tissue. a, brain tissue from the normal mouse group; b, brain tissue from the mouse group subjected to radiotherapy; c, peritumoral brain tissue from the mouse group with tumor induction alone; d, peritumoral brain tissue from the mouse group with tumor induction followed by radiotherapy; e, tumor tissue from the mouse group with tumor induction alone; f, tumor tissue from the mouse group with tumor induction followed by radiotherapy. (D) Bar chart of immunohistochemistry (n=6). (E) Survival time of mice with solely transplanted tumors and mice with transplanted tumors following cranial irradiation after receiving anti-PD-1 therapy (n=6). (F) The MRI scan clearly illustrates the size of the tumor, and the tumor is outlined by a red dashed line. (G) Kill rate analysis of GL261 glioma cells co-cultured with CD8 + T cells. Scale bars: 40 µm for IHC images. ns, not significant; *, P<0.05; **, P<0.01; ***, P<0.001. IHC, immunohistochemistry; MRI, magnetic resonance imaging; PD-1, programmed cell death protein 1.
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    Image Search Results


    Intracranial tumor transplantation experiment. (A) Survival duration of normal mice, mice subjected to cranial irradiation, mice with solely transplanted tumors, and mice with transplanted tumors following cranial irradiation (n=6). (B) The MRI scan clearly illustrates the size of the tumor, and the tumor is outlined by a red dashed line. (C) IHC of CD4, CD8, CD56, PD1, and Ki67 in peritumoral tissue and tumor tissue. a, brain tissue from the normal mouse group; b, brain tissue from the mouse group subjected to radiotherapy; c, peritumoral brain tissue from the mouse group with tumor induction alone; d, peritumoral brain tissue from the mouse group with tumor induction followed by radiotherapy; e, tumor tissue from the mouse group with tumor induction alone; f, tumor tissue from the mouse group with tumor induction followed by radiotherapy. (D) Bar chart of immunohistochemistry (n=6). (E) Survival time of mice with solely transplanted tumors and mice with transplanted tumors following cranial irradiation after receiving anti-PD-1 therapy (n=6). (F) The MRI scan clearly illustrates the size of the tumor, and the tumor is outlined by a red dashed line. (G) Kill rate analysis of GL261 glioma cells co-cultured with CD8 + T cells. Scale bars: 40 µm for IHC images. ns, not significant; *, P<0.05; **, P<0.01; ***, P<0.001. IHC, immunohistochemistry; MRI, magnetic resonance imaging; PD-1, programmed cell death protein 1.

    Journal: Translational Cancer Research

    Article Title: Radiation-activated astrocytes promote tumor progression through CXCL12 secretion and induce resistance to anti-PD-1 immunotherapy

    doi: 10.21037/tcr-2025-1-2652

    Figure Lengend Snippet: Intracranial tumor transplantation experiment. (A) Survival duration of normal mice, mice subjected to cranial irradiation, mice with solely transplanted tumors, and mice with transplanted tumors following cranial irradiation (n=6). (B) The MRI scan clearly illustrates the size of the tumor, and the tumor is outlined by a red dashed line. (C) IHC of CD4, CD8, CD56, PD1, and Ki67 in peritumoral tissue and tumor tissue. a, brain tissue from the normal mouse group; b, brain tissue from the mouse group subjected to radiotherapy; c, peritumoral brain tissue from the mouse group with tumor induction alone; d, peritumoral brain tissue from the mouse group with tumor induction followed by radiotherapy; e, tumor tissue from the mouse group with tumor induction alone; f, tumor tissue from the mouse group with tumor induction followed by radiotherapy. (D) Bar chart of immunohistochemistry (n=6). (E) Survival time of mice with solely transplanted tumors and mice with transplanted tumors following cranial irradiation after receiving anti-PD-1 therapy (n=6). (F) The MRI scan clearly illustrates the size of the tumor, and the tumor is outlined by a red dashed line. (G) Kill rate analysis of GL261 glioma cells co-cultured with CD8 + T cells. Scale bars: 40 µm for IHC images. ns, not significant; *, P<0.05; **, P<0.01; ***, P<0.001. IHC, immunohistochemistry; MRI, magnetic resonance imaging; PD-1, programmed cell death protein 1.

    Article Snippet: First, CD8 + T cells were cultured in CD8 + T cell complete medium (Procell, CM-M380) and then activated using IL-2 and CD3/CD28 antibodies.

    Techniques: Transplantation Assay, Irradiation, Immunohistochemistry, Cell Culture, Magnetic Resonance Imaging